Hotspot

ndhD-psaC

SSC · 115573-115668 · 96 bp

Back to Antirrhinum

Pi 0.0573

MarkerSeek score 63.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 96
variable_sites: 11
pi: 0.0573
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GCAAGGAAAATGGTTCGTGG	AAGAGATGTGAATCCGCCTG	50.0	50.0	57.9	57.7	234 bp	1.0	80.7
ndhD-psaC_p2	GCAAGGAAAATGGTTCGTGG	TGAAACAACCCGCAGTATGG	50.0	50.0	57.9	58.5	175 bp	1.0	80.7
ndhD-psaC_p3	GCAAGGAAAATGGTTCGTGG	CGAGGACTGTGTTGGTTGTA	50.0	50.0	57.9	57.5	253 bp	1.0	78.8
ndhD-psaC_p4	GCAAGGAAAATGGTTCGTGG	GCATGAAACAACCCGCAGTA	50.0	50.0	57.9	59.1	178 bp	1.0	75.5
ndhD-psaC_p5	GCAAGGAAAATGGTTCGTGG	CATGAAACAACCCGCAGTATG	50.0	47.6	57.9	57.9	177 bp	1.0	75.4