Hotspot

rps19-rpl2

IRb · 83084-83162 · 79 bp

Back to Antirrhinum

Pi 0.0241
MarkerSeek score 52.70
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
IRb
length_bp
79
variable_sites
3
pi
0.0241
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 AGATGATTGGCTACAAAAGGA GATTGTTCGTCGCCGTAGTA 38.1 50.0 54.9 57.8 140-148 bp 1.0 51.3
rps19-rpl2_p2 AGATGATTGGCTACAAAAGGA TTTGATTGTTCGTCGCCGTA 38.1 45.0 54.9 58.2 143-151 bp 1.0 51.0
rps19-rpl2_p3 AGATGATTGGCTACAAAAGGA CACAACCCCTTGGGGTTATC 38.1 55.0 54.9 58.2 209-217 bp 1.0 50.0
rps19-rpl2_p4 AGATGATTGGCTACAAAAGGA TTGGGGTTATCCTGCACTTG 38.1 50.0 54.9 57.8 200-208 bp 1.0 49.7
rps19-rpl2_p5 AGATGATTGGCTACAAAAGGA TTGTTCGTCGCCGTAGTAAG 38.1 50.0 54.9 58.0 146 bp 0.25 16.8