Hotspot

psaI-ycf4

LSC · 58615-59069 · 455 bp

Back to Antirrhinum

Pi 0.0033

MarkerSeek score 55.90

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 3
pi: 0.0033
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TAGGCCTAGTATTTCCGGCA	AGGGCCCAGCAAAAATTACT	50.0	45.0	57.9	57.7	603 bp	1.0	77.1
psaI-ycf4_p2	AGTATTTCCGGCACTTGCAA	AGGGCCCAGCAAAAATTACT	45.0	45.0	58.1	57.7	596 bp	1.0	77.0
psaI-ycf4_p3	GCACTTGCAATGGCTTCTTT	AGGGCCCAGCAAAAATTACT	45.0	45.0	57.8	57.7	586 bp	1.0	76.5
psaI-ycf4_p4	GCCTAGTATTTCCGGCACTT	AGGGCCCAGCAAAAATTACT	50.0	45.0	57.7	57.7	600 bp	1.0	75.2
psaI-ycf4_p5	GTAGGCCTAGTATTTCCGGC	AGGGCCCAGCAAAAATTACT	55.0	45.0	57.5	57.7	604 bp	1.0	74.2