Hotspot

rbcL-accD

LSC · 55691-56377 · 687 bp

Back to Antirrhinum

Pi 0.00315

MarkerSeek score 46.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 4
pi: 0.00315
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	CATCGAATTAACCCACCACCT	50.0	47.6	57.9	58.0	780 bp	1.0	71.5
rbcL-accD_p2	CCGTGAAGCTAGCAAATGGA	CATCGAATTAACCCACCACCT	50.0	47.6	58.3	58.0	814 bp	1.0	70.3
rbcL-accD_p3	TCCGTGAAGCTAGCAAATGG	CATCGAATTAACCCACCACCT	50.0	47.6	58.3	58.0	815 bp	1.0	70.3
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	CATCGAATTAACCCACCACCT	55.0	47.6	57.7	58.0	796 bp	1.0	70.1
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	ACCATCGAATTAACCCACCA	50.0	45.0	57.9	56.8	782 bp	1.0	70.0