Hotspot

rpoB-trnC-GCA

LSC · 26555-27748 · 1194 bp

Back to Antirrhinum

Pi 0.00558

MarkerSeek score 51.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1194
variable_sites: 13
pi: 0.00558
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTGCCTCATTTGCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1274-1283 bp	1.0	79.2
rpoB-trnC-GCA_p2	CATTGCCTCATTTGCATCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.8	58.4	1256-1265 bp	1.0	77.1
rpoB-trnC-GCA_p3	ATTGCCTCATTTGCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.9	58.1	1273-1282 bp	1.0	74.1
rpoB-trnC-GCA_p4	CATTGCCTCATTTGCATCCC	TTTGTTGATCAGGCGACACC	50.0	50.0	57.8	58.8	1293-1302 bp	1.0	73.7
rpoB-trnC-GCA_p5	ACATTGCCTCATTTGCATCC	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.0	58.1	1275-1284 bp	1.0	73.3