Hotspot

trnS-GCU-trnG-UCC

LSC · 8168-8824 · 657 bp

Back to Antirrhinum

Pi 0.00634

MarkerSeek score 55.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 657
variable_sites: 8
pi: 0.00634
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AACCGAAAGACCCTTTAACT	50.0	40.0	58.0	54.5	763 bp	1.0	52.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	AACCGAAAGACCCTTTAACT	50.0	40.0	58.0	54.5	762 bp	1.0	52.7
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	726 bp	1.0	52.6
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	725 bp	1.0	52.5
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	AACCGAAAGACCCTTTAACT	50.0	40.0	58.2	54.5	817 bp	1.0	51.5