Hotspot

psbI-trnS-GCU

LSC · 7960-8079 · 120 bp

Back to Antirrhinum

Pi 0.0042

MarkerSeek score 53.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 120
variable_sites: 1
pi: 0.0042
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATCTAATGATCCGGGGCGTA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	231-238 bp	1.0	80.4
psbI-trnS-GCU_p2	TATCTAATGATCCGGGGCGT	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	232-239 bp	1.0	80.4
psbI-trnS-GCU_p3	ATCTAATGATCCGGGGCGTA	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	232-239 bp	1.0	80.4
psbI-trnS-GCU_p4	TATCTAATGATCCGGGGCGT	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	233-240 bp	1.0	80.4
psbI-trnS-GCU_p5	TCTAATGATCCGGGGCGTAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.3	58.0	230-237 bp	1.0	78.6