Hotspot

ycf1

SSC · 121794-127142 · 5349 bp

Back to Anthyllis

Pi 0.00262

MarkerSeek score 48.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5349
variable_sites: 21
pi: 0.00262
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCCCGAATCCCGTAAGAGT	TCGGACAGTAAAAAGGGTGA	50.0	45.0	58.1	56.4	281 bp	1.0	66.6
ycf1_p2	TCCCGTAAGAGTGGAATCCT	TCGGACAGTAAAAAGGGTGA	50.0	45.0	57.5	56.4	273 bp	1.0	63.4
ycf1_p3	TTCCCGAATCCCGTAAGAGT	ATCGGACAGTAAAAAGGGTGA	50.0	42.9	58.1	56.9	282 bp	1.0	62.9
ycf1_p4	TTCCCGAATCCCGTAAGAGT	TCGGACAGTAAAAAGGGTGAT	50.0	42.9	58.1	56.9	281 bp	1.0	62.9
ycf1_p5	CCGAATCCCGTAAGAGTGGA	TCGGACAGTAAAAAGGGTGA	55.0	45.0	58.9	56.4	278 bp	1.0	60.7