Hotspot

rps18

LSC · 67022-67339 · 318 bp

Back to Anthyllis

Pi 0.0021

MarkerSeek score 28.70

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 318
variable_sites: 1
pi: 0.0021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AACGCTTGTGTGTCACTCTT	ACAACACAACAACCCCCAAT	45.0	45.0	58.0	58.1	641-645 bp	1.0	80.2
rps18_p2	AACGCTTGTGTGTCACTCTT	TTACATCAAGCTTTCGCGGA	45.0	45.0	58.0	57.9	1104-1108 bp	1.0	80.1
rps18_p3	TCTGTCCTTGTTGTCGCAAA	TTACATCAAGCTTTCGCGGA	45.0	45.0	58.0	57.9	1166-1170 bp	1.0	80.0
rps18_p4	TTCTGTCCTTGTTGTCGCAA	TTACATCAAGCTTTCGCGGA	45.0	45.0	58.0	57.9	1167-1171 bp	1.0	80.0
rps18_p5	GCTTGTGTGTCACTCTTCCA	TTACATCAAGCTTTCGCGGA	50.0	45.0	58.0	57.9	1101-1105 bp	1.0	79.9