Hotspot

atpB

LSC · 7418-8914 · 1497 bp

Back to Anthyllis

Pi 0.00134

MarkerSeek score 59.60

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 3
pi: 0.00134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	ACCGATCAACTTTCTTTGTTATCG	TTTCCTTCACGTCGGAATCC	37.5	50.0	57.8	57.9	1636 bp	1.0	46.1
atpB_p2	ACCGATCAACTTTCTTTGTTATCG	CATAGGCGCATGATTGGGTA	37.5	50.0	57.8	57.8	1692 bp	1.0	45.3
atpB_p3	ACCGATCAACTTTCTTTGTTATCG	ACGTCGGAATCCCAAACAAT	37.5	45.0	57.8	57.8	1628 bp	1.0	45.3
atpB_p4	ACCGATCAACTTTCTTTGTTATCG	CTTCACGTCGGAATCCCAAA	37.5	50.0	57.8	58.2	1632 bp	1.0	45.2
atpB_p5	CCGATCAACTTTCTTTGTTATCGA	TTTCCTTCACGTCGGAATCC	37.5	50.0	57.6	57.9	1635 bp	1.0	44.2