Hotspot

ndhD-psaC

SSC · 114799-114933 · 135 bp

Back to Anthyllis

Pi 0.00995
MarkerSeek score 59.50
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
135
variable_sites
2
pi
0.00995
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 ACCACCTAATTACCTTATTTCCCC GCTTGTCCGACGGATTTCTT 41.7 50.0 58.0 58.6 315-316 bp 1.0 44.1
ndhD-psaC_p2 CCACCTAATTACCTTATTTCCCCT GCTTGTCCGACGGATTTCTT 41.7 50.0 57.7 58.6 314-315 bp 1.0 42.1
ndhD-psaC_p3 ACCACCTAATTACCTTATTTCCCC TGTTGGTTGTAAAAGGTGCG 41.7 45.0 58.0 57.1 340-341 bp 1.0 41.7
ndhD-psaC_p4 ACCACCTAATTACCTTATTTCCCC GCTTGTCCGACGGATTTCT 41.7 52.6 58.0 57.9 315-316 bp 1.0 40.8
ndhD-psaC_p5 CCACCTAATTACCTTATTTCCCCT TGTTGGTTGTAAAAGGTGCG 41.7 45.0 57.7 57.1 339-340 bp 1.0 40.7