Hotspot

rps12-clpP1

LSC · 68927-69096 · 170 bp

Back to Anthyllis

Pi 0.0118

MarkerSeek score 60.60

Primer available yes

Label rps12-clpP1

Feature Metadata

label_name: rps12-clpP1
feature_type: igs
region: LSC
length_bp: 170
variable_sites: 3
pi: 0.0118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP1_p1	TGACATTTCGGATTGGCTGT	AGCCCAAGCCTATGGAATTG	45.0	50.0	57.8	57.9	267-268 bp	1.0	79.0
rps12-clpP1_p2	CTAGTACAAGTTCCTCGGCG	AGCCCAAGCCTATGGAATTG	55.0	50.0	57.8	57.9	319-320 bp	1.0	78.9
rps12-clpP1_p3	TTTCGGATTGGCTGTCTTGT	AGCCCAAGCCTATGGAATTG	45.0	50.0	57.7	57.9	262-263 bp	1.0	78.4
rps12-clpP1_p4	TGACATTTCGGATTGGCTGT	GCCCAAGCCTATGGAATTGT	45.0	50.0	57.8	58.2	266-267 bp	1.0	77.9
rps12-clpP1_p5	CTAGTACAAGTTCCTCGGCG	GCCCAAGCCTATGGAATTGT	55.0	50.0	57.8	58.2	318-319 bp	1.0	77.7