Hotspot

rpl20-rps12

LSC · 67967-68812 · 846 bp

Back to Anthyllis

Pi 0.00236

MarkerSeek score 47.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 3
pi: 0.00236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CCTCCGTTTTCGCGCTATAT	CGCCGAGGAACTTGTACTAG	50.0	55.0	58.2	57.8	915 bp	1.0	77.0
rpl20-rps12_p2	CCTCCGTTTTCGCGCTATAT	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.2	57.8	967 bp	1.0	76.9
rpl20-rps12_p3	AAGTCTTGAATGAGCTCCGC	CGCCGAGGAACTTGTACTAG	50.0	55.0	58.3	57.8	966 bp	1.0	76.6
rpl20-rps12_p4	AAGTCTTGAATGAGCTCCGC	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.3	57.8	1018 bp	1.0	76.5
rpl20-rps12_p5	CCTCCGTTTTCGCGCTATAT	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.2	57.7	972 bp	1.0	76.3