Hotspot

trnQ-UUG-rps16

LSC · 55490-56020 · 531 bp

Back to Anthyllis

Pi 0.00502

MarkerSeek score 55.00

Primer available yes

Label trnQ-UUG-rps16

Feature Metadata

label_name: trnQ-UUG-rps16
feature_type: igs
region: LSC
length_bp: 531
variable_sites: 4
pi: 0.00502
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-rps16_p1	GAATCAAAAATGGGGCGTGG	GTCTTTCAAGTCGCACGTTG	50.0	50.0	58.0	58.0	669-681 bp	1.0	81.2
trnQ-UUG-rps16_p2	GAATCAAAAATGGGGCGTGG	AATGGCGGATGTCAGAATCC	50.0	50.0	58.0	58.0	707-719 bp	1.0	81.0
trnQ-UUG-rps16_p3	GAGGTTCGAATCCTTCCGTC	GTCTTTCAAGTCGCACGTTG	55.0	50.0	58.1	58.0	611-623 bp	1.0	80.8
trnQ-UUG-rps16_p4	GTCGAATCAAAAATGGGGCG	GTCTTTCAAGTCGCACGTTG	50.0	50.0	58.1	58.0	672-684 bp	1.0	80.5
trnQ-UUG-rps16_p5	GAGGTTCGAATCCTTCCGTC	AATGGCGGATGTCAGAATCC	55.0	50.0	58.1	58.0	649-661 bp	1.0	80.5