Hotspot

trnT-GGU-trnE-UUC

LSC · 29980-30808 · 829 bp

Back to Anthyllis

Pi 0.00322

MarkerSeek score 60.80

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 4
pi: 0.00322
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	TTTCGTAGTACCCTACCCCC	50.0	55.0	58.0	57.8	970-971 bp	1.0	79.0
trnT-GGU-trnE-UUC_p2	GAACCGATGACTTACGCCTT	CGTTGCCTCCTTGAAAGAGA	50.0	50.0	58.0	57.8	933-934 bp	1.0	78.9
trnT-GGU-trnE-UUC_p3	CCATGGCGTTACTCTACCAC	TTTCGTAGTACCCTACCCCC	55.0	55.0	58.1	57.8	949-950 bp	1.0	78.6
trnT-GGU-trnE-UUC_p4	CCATGGCGTTACTCTACCAC	CGTTGCCTCCTTGAAAGAGA	55.0	50.0	58.1	57.8	912-913 bp	1.0	78.5
trnT-GGU-trnE-UUC_p5	TGGCGTTACTCTACCACTGA	TTTCGTAGTACCCTACCCCC	50.0	55.0	58.1	57.8	946-947 bp	1.0	78.4