Hotspot

trnL-UAA-trnT-UGU

LSC · 13945-14831 · 887 bp

Back to Anthyllis

Pi 0.00414

MarkerSeek score 61.70

Primer available yes

Label trnL-UAA-trnT-UGU

Feature Metadata

label_name: trnL-UAA-trnT-UGU
feature_type: igs
region: LSC
length_bp: 887
variable_sites: 5
pi: 0.00414
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT-UGU_p1	CCCATTGTGAATTCCAGGGT	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.8	57.9	1030-1089 bp	1.0	77.7
trnL-UAA-trnT-UGU_p2	CCGTAGCGTCTACCGATTTC	GCGATGCTCTAACCTCTGAG	55.0	55.0	58.2	57.9	948-1007 bp	1.0	77.6
trnL-UAA-trnT-UGU_p3	CCCATTGTGAATTCCAGGGT	GGAATCGAACCGATGACCAT	50.0	50.0	57.8	57.8	1062-1121 bp	1.0	76.8
trnL-UAA-trnT-UGU_p4	CCGTAGCGTCTACCGATTTC	GGAATCGAACCGATGACCAT	55.0	50.0	58.2	57.8	980-1039 bp	1.0	76.7
trnL-UAA-trnT-UGU_p5	CGTCTACCGATTTCGCCATA	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.6	57.9	942-1001 bp	1.0	76.3