Hotspot

ndhJ-trnF-GAA

LSC · 12461-13099 · 639 bp

Back to Anthyllis

Pi 0.00316
MarkerSeek score 66.00
Primer available yes
Label ndhJ-trnF-GAA

Feature Metadata

label_name
ndhJ-trnF-GAA
feature_type
igs
region
LSC
length_bp
639
variable_sites
3
pi
0.00316
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-trnF-GAA_p1 AATGCCCGAAAGCTGGATAG GGGATAGCTCAGCTGGTAGA 50.0 55.0 58.0 58.0 747-791 bp 1.0 84.1
ndhJ-trnF-GAA_p2 AATGCCCGAAAGCTGGATAG CCTCGTGTCACCAGTTCAAA 50.0 50.0 58.0 58.1 711-755 bp 1.0 83.7
ndhJ-trnF-GAA_p3 AATGCCCGAAAGCTGGATAG AGAATGGTCGGGATAGCTCA 50.0 50.0 58.0 57.9 756-800 bp 1.0 83.4
ndhJ-trnF-GAA_p4 AATGCCCGAAAGCTGGATAG CTGGTAGAGCAGAGGACTGA 50.0 55.0 58.0 57.9 735-779 bp 1.0 83.0
ndhJ-trnF-GAA_p5 TTTAATGCCCGAAAGCTGGA GGGATAGCTCAGCTGGTAGA 45.0 55.0 57.8 58.0 750-794 bp 1.0 82.6