Hotspot

ycf1

SSC · 131394-137078 · 5685 bp

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Pi 0.445

MarkerSeek score 74.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5685
variable_sites: 2099
pi: 0.445
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTTACCCATCCAACCCCT	ACACGACCGAAAAGCTATCC	50.0	50.0	57.9	58.0	1042 bp	0.5	59.4
ycf1_p2	AAGTGGCGAATGCACATTTG	ACACGACCGAAAAGCTATCC	45.0	50.0	57.9	58.0	953 bp	0.5	59.4
ycf1_p3	CAAATCCTCGTACCACTCGG	ACACGACCGAAAAGCTATCC	55.0	50.0	58.1	58.0	1301 bp	0.5	59.3
ycf1_p4	TCTTTACCCATCCAACCCCT	TGCAGAGGGGTCCATAGAAA	50.0	50.0	57.9	58.0	756 bp	0.5	59.2
ycf1_p5	AAGTGGCGAATGCACATTTG	TGCAGAGGGGTCCATAGAAA	45.0	50.0	57.9	58.0	667 bp	0.5	59.2