Hotspot

rps15

SSC · 130113-130391 · 279 bp

Back to Anthurium

Pi 0.525

MarkerSeek score 73.50

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 279
variable_sites: 145
pi: 0.525
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	CCAATAATGCCCACTCCCTC	AGTGCGTGAGTGAGACATTC	55.0	50.0	58.0	57.9	1410 bp	0.5	59.3
rps15_p2	TATTGCTTCCTCTCCCCCAA	AGTGCGTGAGTGAGACATTC	50.0	50.0	58.0	57.9	1426 bp	0.5	59.3
rps15_p3	TGCTTCCTCTCCCCCAATAA	AGTGCGTGAGTGAGACATTC	50.0	50.0	58.0	57.9	1423 bp	0.5	59.3
rps15_p4	TTATTGCTTCCTCTCCCCCA	AGTGCGTGAGTGAGACATTC	50.0	50.0	58.0	57.9	1427 bp	0.5	59.3
rps15_p5	TTGCTTCCTCTCCCCCAATA	AGTGCGTGAGTGAGACATTC	50.0	50.0	58.0	57.9	1424 bp	0.5	59.3