Hotspot

psaC

SSC · 122267-122512 · 246 bp

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Pi 0.451

MarkerSeek score 75.00

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 96
pi: 0.451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	AACGGGTCGTCCAATTGAAA	AACCACTGATTCGTCTGGTG	45.0	50.0	58.0	57.8	662 bp	0.5	58.4
psaC_p2	ACGGGTCGTCCAATTGAAAA	AACCACTGATTCGTCTGGTG	45.0	50.0	58.0	57.8	661 bp	0.5	58.4
psaC_p3	AACGGGTCGTCCAATTGAAA	CCTTGGCCCTTTTCTCATGA	45.0	50.0	58.0	57.8	729 bp	0.5	58.0
psaC_p4	ACGGGTCGTCCAATTGAAAA	CCTTGGCCCTTTTCTCATGA	45.0	50.0	58.0	57.8	728 bp	0.5	58.0
psaC_p5	ACGGGTCGTCCAATTGAAAA	AATCCCTTGGCCCTTTTCTC	45.0	50.0	58.0	57.8	732 bp	0.5	57.9