Hotspot

ndhH

SSC · 128827-130008 · 1182 bp

Back to Anthurium

Pi 0.477

MarkerSeek score 81.80

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 498
pi: 0.477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TATGACCGCTATTGTTGGCC	TATTCGGGAGCCAAAAACCC	50.0	50.0	58.0	58.2	1633 bp	0.5	58.4
ndhH_p2	TCCCTAATGGACCGGCATAT	TATTCGGGAGCCAAAAACCC	50.0	50.0	58.0	58.2	1526 bp	0.5	58.4
ndhH_p3	ATTCAGGTCCAATGCGTTGT	TATTCGGGAGCCAAAAACCC	45.0	50.0	58.1	58.2	1508 bp	0.5	58.0
ndhH_p4	AGGTCCAATGCGTTGTTGTA	TATTCGGGAGCCAAAAACCC	45.0	50.0	57.7	58.2	1504 bp	0.5	56.6
ndhH_p5	CCGGCATATTCAGGTCCAAT	TATTCGGGAGCCAAAAACCC	50.0	50.0	57.7	58.2	1515 bp	0.5	56.5