Hotspot

ndhF

SSC · 114997-117234 · 2238 bp

Back to Anthurium

Pi 0.463

MarkerSeek score 77.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 803
pi: 0.463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGACTTCATTTTTCACTTTTT	ATTAGCGGATACTGGGTCCA	23.8	50.0	50.3	57.9	2736 bp	0.5	20.0
ndhF_p2	TGACTTCATTTTTCACTTTTT	TTAGCGGATACTGGGTCCAT	23.8	50.0	50.3	57.9	2735 bp	0.5	20.0
ndhF_p3	TTGACTTCATTTTTCACTTTT	ATTAGCGGATACTGGGTCCA	23.8	50.0	50.3	57.9	2737 bp	0.5	20.0
ndhF_p4	TTGACTTCATTTTTCACTTTT	TTAGCGGATACTGGGTCCAT	23.8	50.0	50.3	57.9	2736 bp	0.5	20.0
ndhF_p5	TTTGACTTCATTTTTCACTTT	TTAGCGGATACTGGGTCCAT	23.8	50.0	50.3	57.9	2737 bp	0.5	20.0