Hotspot

ndhA

SSC · 126643-128825 · 2183 bp

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Pi 0.44

MarkerSeek score 76.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2183
variable_sites: 724
pi: 0.44
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGCAGCTCGTACTGTTTGTT	CAATGTTACGAGCTTCCGGA	45.0	50.0	58.1	58.0	2852 bp	0.5	59.5
ndhA_p2	TTGCAGCTCGTACTGTTTGT	CAATGTTACGAGCTTCCGGA	45.0	50.0	58.1	58.0	2853 bp	0.5	59.5
ndhA_p3	TGCAGCTCGTACTGTTTGTT	GAGGGAGTGGGCATTATTGG	45.0	55.0	58.1	58.0	2907 bp	0.5	59.5
ndhA_p4	TGCAGCTCGTACTGTTTGTT	GGGGCTTATCAGGACCAATG	45.0	55.0	58.1	58.0	2867 bp	0.5	59.5
ndhA_p5	TTGCAGCTCGTACTGTTTGT	GGGGCTTATCAGGACCAATG	45.0	55.0	58.1	58.0	2868 bp	0.5	59.5