Hotspot

rps15-ycf1

SSC · 130392-131393 · 1002 bp

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Pi 0.441

MarkerSeek score 79.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 1002
variable_sites: 361
pi: 0.441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ATCAGTCGAAGAACACCGTG	CTAGACGTCGCAGAGAGTTG	50.0	55.0	57.9	57.8	1782 bp	0.5	58.1
rps15-ycf1_p2	TTCCCAATTGTTCTGGTGCA	CTAGACGTCGCAGAGAGTTG	45.0	55.0	57.9	57.8	1967 bp	0.5	58.0
rps15-ycf1_p3	ATCAGTCGAAGAACACCGTG	ACTAGACGTCGCAGAGAGTT	50.0	50.0	57.9	58.2	1783 bp	0.5	58.0
rps15-ycf1_p4	ATCAGTCGAAGAACACCGTG	TGAACCGGTTCGTCTCTCTA	50.0	50.0	57.9	57.8	2009 bp	0.5	58.0
rps15-ycf1_p5	ATCAGTCGAAGAACACCGTG	TAGACGTCGCAGAGAGTTGA	50.0	50.0	57.9	58.2	1781 bp	0.5	57.9