Hotspot

ndhI-ndhA

SSC · 126550-126642 · 93 bp

Back to Anthurium

Pi 0.5

MarkerSeek score 75.80

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 93
variable_sites: 45
pi: 0.5
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TGCAGCTCGTACTGTTTGTT	ACCAGAAGCAGAGGAGGAAT	45.0	50.0	58.1	58.0	544 bp	0.5	59.3
ndhI-ndhA_p2	TTGCAGCTCGTACTGTTTGT	ACCAGAAGCAGAGGAGGAAT	45.0	50.0	58.1	58.0	545 bp	0.5	59.3
ndhI-ndhA_p3	TGCAGCTCGTACTGTTTGTT	TTGGGGGTGGAATTTATGGC	45.0	50.0	58.1	58.1	634 bp	0.5	58.6
ndhI-ndhA_p4	TTGCAGCTCGTACTGTTTGT	TTGGGGGTGGAATTTATGGC	45.0	50.0	58.1	58.1	635 bp	0.5	58.6
ndhI-ndhA_p5	TGCAGCTCGTACTGTTTGTT	TGGTTTTTGGGGGTGGAATT	45.0	45.0	58.1	57.8	640 bp	0.5	58.0