Hotspot

ndhG-ndhI

SSC · 125302-126006 · 705 bp

Back to Anthurium

Pi 0.326

MarkerSeek score 82.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 705
variable_sites: 207
pi: 0.326
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TACAAACCAGCGAAGTGACC	AACAAACAGTACGAGCTGCA	50.0	45.0	58.1	58.1	1524 bp	0.5	58.6
ndhG-ndhI_p2	TACAAACCAGCGAAGTGACC	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.1	58.1	1523 bp	0.5	58.6
ndhG-ndhI_p3	TGTGCGGCAGCTACAAAATA	AACAAACAGTACGAGCTGCA	45.0	45.0	58.2	58.1	1396 bp	0.5	58.1
ndhG-ndhI_p4	TGTGCGGCAGCTACAAAATA	ACAAACAGTACGAGCTGCAA	45.0	45.0	58.2	58.1	1395 bp	0.5	58.1
ndhG-ndhI_p5	CACGGTCCAAAGAGGGAAAT	ACAAACAGTACGAGCTGCAA	50.0	45.0	57.8	58.1	1495 bp	0.5	58.0