Hotspot

ndhE-ndhG

SSC · 124385-124770 · 386 bp

Back to Anthurium

Pi 0.435

MarkerSeek score 74.40

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 386
variable_sites: 130
pi: 0.435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GTGCTCTAACCATGCTTCGA	GGTCACTTCGCTGGTTTGTA	50.0	50.0	58.0	58.1	715 bp	0.5	58.9
ndhE-ndhG_p2	TGCTCTAACCATGCTTCGAC	GGTCACTTCGCTGGTTTGTA	50.0	50.0	58.0	58.1	714 bp	0.5	58.9
ndhE-ndhG_p3	AGTGCTCTAACCATGCTTCG	GGTCACTTCGCTGGTTTGTA	50.0	50.0	58.0	58.1	716 bp	0.5	58.9
ndhE-ndhG_p4	CGCGTCGATTTTCTGTTACG	GGTCACTTCGCTGGTTTGTA	50.0	50.0	58.0	58.1	899 bp	0.5	58.7
ndhE-ndhG_p5	TGCTCTAACCATGCTTCGAC	TATTTTGTAGCTGCCGCACA	50.0	45.0	58.0	58.2	842 bp	0.5	58.4