Hotspot

psaC-ndhE

SSC · 122513-124078 · 1566 bp

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Pi 0.441

MarkerSeek score 83.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 1566
variable_sites: 646
pi: 0.441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACCTACGCAATCTTCGGTTC	GTCGAAGCATGGTTAGAGCA	50.0	50.0	58.0	58.0	1952 bp	0.5	59.9
psaC-ndhE_p2	ACCTACGCAATCTTCGGTTC	TCGAAGCATGGTTAGAGCAC	50.0	50.0	58.0	58.0	1951 bp	0.5	59.9
psaC-ndhE_p3	ACCTACGCAATCTTCGGTTC	CGAAGCATGGTTAGAGCACT	50.0	50.0	58.0	58.0	1950 bp	0.5	59.8
psaC-ndhE_p4	ACGGGTCGTCCAATTGAAAA	GTCGAAGCATGGTTAGAGCA	45.0	50.0	58.0	58.0	2357 bp	0.5	59.7
psaC-ndhE_p5	ACGGGTCGTCCAATTGAAAA	TCGAAGCATGGTTAGAGCAC	45.0	50.0	58.0	58.0	2356 bp	0.5	59.7