Hotspot

rpl32-trnL-UAG

SSC · 118634-119354 · 721 bp

Back to Anthurium

Pi 0.465
MarkerSeek score 82.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
721
variable_sites
302
pi
0.465
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTGTGCGACCAAACGAGTAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 770 bp 0.5 59.4
rpl32-trnL-UAG_p2 TTTGTGCGACCAAACGAGTA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 771 bp 0.5 59.4
rpl32-trnL-UAG_p3 TTGTGCGACCAAACGAGTAA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 771 bp 0.5 59.4
rpl32-trnL-UAG_p4 TTTGTGCGACCAAACGAGTA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 772 bp 0.5 59.4
rpl32-trnL-UAG_p5 TTGTGCGACCAAACGAGTAA GCACTTGGTGTGGTTATTGC 45.0 50.0 58.1 57.9 1224 bp 0.5 59.0