primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	CCTGGCCCAAATCAGCATAT	AAAGGTCGTAACTGGGGGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033932	113908	114996	1817-1817	0.500000	59.7
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	ACCTGGGGCTTTTTACAAGG	AAAGGTCGTAACTGGGGGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038243	113908	114996	1585-1585	0.500000	59.7
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	CGGGAGAATTGATGACTGCA	AAAGGTCGTAACTGGGGGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056981	113908	114996	1663-1663	0.500000	59.5
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	AACGGTAAAAACGAGCCCTT	AAAGGTCGTAACTGGGGGTA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.057080	113908	114996	1447-1447	0.500000	59.5
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	ACGGTAAAAACGAGCCCTTT	AAAGGTCGTAACTGGGGGTA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.057080	113908	114996	1446-1446	0.500000	59.5
ndhF_p1	ndhF	1	TGACTTCATTTTTCACTTTTT	ATTAGCGGATACTGGGTCCA	21	20	23.8	50.0	50.3	57.9	0.0	0.0	8.832690	114997	117234	2736-2736	0.500000	20.0
ndhF_p2	ndhF	2	TGACTTCATTTTTCACTTTTT	TTAGCGGATACTGGGTCCAT	21	20	23.8	50.0	50.3	57.9	0.0	0.0	8.832690	114997	117234	2735-2735	0.500000	20.0
ndhF_p3	ndhF	3	TTGACTTCATTTTTCACTTTT	ATTAGCGGATACTGGGTCCA	21	20	23.8	50.0	50.3	57.9	0.0	0.0	8.832690	114997	117234	2737-2737	0.500000	20.0
ndhF_p4	ndhF	4	TTGACTTCATTTTTCACTTTT	TTAGCGGATACTGGGTCCAT	21	20	23.8	50.0	50.3	57.9	0.0	0.0	8.832690	114997	117234	2736-2736	0.500000	20.0
ndhF_p5	ndhF	5	TTTGACTTCATTTTTCACTTT	TTAGCGGATACTGGGTCCAT	21	20	23.8	50.0	50.3	57.9	0.0	0.0	8.832690	114997	117234	2737-2737	0.500000	20.0
ndhF-rpl32_p1	ndhF-rpl32	1	CTGCAATTGGTCGTGTGAAC	TACTCGTTTGGTCGCACAAA	20	20	50.0	45.0	58.0	58.1	7.7	1.0	0.099278	117235	118474	1912-1912	0.500000	59.2
ndhF-rpl32_p2	ndhF-rpl32	2	CTGCAATTGGTCGTGTGAAC	TTACTCGTTTGGTCGCACAA	20	20	50.0	45.0	58.0	58.1	7.7	0.0	0.099278	117235	118474	1913-1913	0.500000	59.2
ndhF-rpl32_p3	ndhF-rpl32	3	AATAGGGAGGCACACAGAGT	TACTCGTTTGGTCGCACAAA	20	20	50.0	45.0	58.0	58.1	0.0	1.0	0.104434	117235	118474	2096-2096	0.500000	59.2
ndhF-rpl32_p4	ndhF-rpl32	4	AATAGGGAGGCACACAGAGT	TTACTCGTTTGGTCGCACAA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.104434	117235	118474	2097-2097	0.500000	59.2
ndhF-rpl32_p5	ndhF-rpl32	5	AGGAAAAGCCCACATACGAC	TACTCGTTTGGTCGCACAAA	20	20	50.0	45.0	57.9	58.1	0.0	1.0	0.172147	117235	118474	1527-1527	0.500000	58.6
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TTGTGCGACCAAACGAGTAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.070552	118634	119354	770-770	0.500000	59.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTTGTGCGACCAAACGAGTA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.070552	118634	119354	771-771	0.500000	59.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TTGTGCGACCAAACGAGTAA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.071923	118634	119354	771-771	0.500000	59.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTTGTGCGACCAAACGAGTA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.071923	118634	119354	772-772	0.500000	59.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTGTGCGACCAAACGAGTAA	GCACTTGGTGTGGTTATTGC	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.127295	118634	119354	1224-1224	0.500000	59.0
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	TTGCATTGCCAGGAATGAGT	20	20	50.0	45.0	58.0	58.1	0.0	15.1	0.080343	119515	120486	1619-1619	0.500000	59.4
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	TTGCATTGCCAGGAATGAGT	20	20	50.0	45.0	58.0	58.1	0.0	15.1	0.081713	119515	120486	1618-1618	0.500000	59.3
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	CATTGCCAGGAATGAGTGGT	20	20	50.0	50.0	58.0	58.2	0.0	6.0	0.164462	119515	120486	1616-1616	0.500000	58.7
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	CATTGCCAGGAATGAGTGGT	20	20	50.0	50.0	58.0	58.2	0.0	6.0	0.165833	119515	120486	1615-1615	0.500000	58.7
ccsA_p5	ccsA	5	GTGAAATCGGTAGACACGCT	CTTCTCTTGCATTGCCAGGA	20	20	50.0	50.0	58.0	58.2	0.0	9.6	0.177551	119515	120486	1625-1625	0.500000	58.6
ndhD_p1	ndhD	1	TCTCTCTGGAGCAGTATGGG	GAACCGAAGATTGCGTAGGT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.085140	120636	122135	2146-2146	0.500000	59.3
ndhD_p2	ndhD	2	TCTCTCTGGAGCAGTATGGG	TATGGCATGAGACAACTCGC	20	20	55.0	50.0	57.9	58.1	0.0	11.0	0.139195	120636	122135	2071-2071	0.500000	58.9
ndhD_p3	ndhD	3	AATGAGGCATGGGGATCCTA	GAACCGAAGATTGCGTAGGT	20	20	50.0	50.0	57.9	58.0	12.6	0.0	0.144505	120636	122135	2124-2124	0.500000	58.8
ndhD_p4	ndhD	4	TAATGAGGCATGGGGATCCT	GAACCGAAGATTGCGTAGGT	20	20	50.0	50.0	57.9	58.0	12.6	0.0	0.144505	120636	122135	2125-2125	0.500000	58.8
ndhD_p5	ndhD	5	TGAGGCATGGGGATCCTATT	GAACCGAAGATTGCGTAGGT	20	20	50.0	50.0	57.9	58.0	12.6	0.0	0.144505	120636	122135	2122-2122	0.500000	58.8
psaC_p1	psaC	1	AACGGGTCGTCCAATTGAAA	AACCACTGATTCGTCTGGTG	20	20	45.0	50.0	58.0	57.8	7.1	0.0	0.199741	122267	122512	662-662	0.500000	58.4
psaC_p2	psaC	2	ACGGGTCGTCCAATTGAAAA	AACCACTGATTCGTCTGGTG	20	20	45.0	50.0	58.0	57.8	7.1	0.0	0.199741	122267	122512	661-661	0.500000	58.4
psaC_p3	psaC	3	AACGGGTCGTCCAATTGAAA	CCTTGGCCCTTTTCTCATGA	20	20	45.0	50.0	58.0	57.8	7.1	0.0	0.250561	122267	122512	729-729	0.500000	58.0
psaC_p4	psaC	4	ACGGGTCGTCCAATTGAAAA	CCTTGGCCCTTTTCTCATGA	20	20	45.0	50.0	58.0	57.8	7.1	0.0	0.250561	122267	122512	728-728	0.500000	58.0
psaC_p5	psaC	5	ACGGGTCGTCCAATTGAAAA	AATCCCTTGGCCCTTTTCTC	20	20	45.0	50.0	58.0	57.8	7.1	0.0	0.267992	122267	122512	732-732	0.500000	57.9
psaC-ndhE_p1	psaC-ndhE	1	ACCTACGCAATCTTCGGTTC	GTCGAAGCATGGTTAGAGCA	20	20	50.0	50.0	58.0	58.0	0.0	18.9	0.016116	122513	124078	1952-1952	0.500000	59.9
psaC-ndhE_p2	psaC-ndhE	2	ACCTACGCAATCTTCGGTTC	TCGAAGCATGGTTAGAGCAC	20	20	50.0	50.0	58.0	58.0	0.0	15.3	0.016116	122513	124078	1951-1951	0.500000	59.9
psaC-ndhE_p3	psaC-ndhE	3	ACCTACGCAATCTTCGGTTC	CGAAGCATGGTTAGAGCACT	20	20	50.0	50.0	58.0	58.0	0.0	10.7	0.021706	122513	124078	1950-1950	0.500000	59.8
psaC-ndhE_p4	psaC-ndhE	4	ACGGGTCGTCCAATTGAAAA	GTCGAAGCATGGTTAGAGCA	20	20	45.0	50.0	58.0	58.0	7.1	18.9	0.040053	122513	124078	2357-2357	0.500000	59.7
psaC-ndhE_p5	psaC-ndhE	5	ACGGGTCGTCCAATTGAAAA	TCGAAGCATGGTTAGAGCAC	20	20	45.0	50.0	58.0	58.0	7.1	15.3	0.040053	122513	124078	2356-2356	0.500000	59.7
ndhE-ndhG_p1	ndhE-ndhG	1	GTGCTCTAACCATGCTTCGA	GGTCACTTCGCTGGTTTGTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136447	124385	124770	715-715	0.500000	58.9
ndhE-ndhG_p2	ndhE-ndhG	2	TGCTCTAACCATGCTTCGAC	GGTCACTTCGCTGGTTTGTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136447	124385	124770	714-714	0.500000	58.9
ndhE-ndhG_p3	ndhE-ndhG	3	AGTGCTCTAACCATGCTTCG	GGTCACTTCGCTGGTTTGTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.142038	124385	124770	716-716	0.500000	58.9
ndhE-ndhG_p4	ndhE-ndhG	4	CGCGTCGATTTTCTGTTACG	GGTCACTTCGCTGGTTTGTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.166513	124385	124770	899-899	0.500000	58.7
ndhE-ndhG_p5	ndhE-ndhG	5	TGCTCTAACCATGCTTCGAC	TATTTTGTAGCTGCCGCACA	20	20	50.0	45.0	58.0	58.2	0.0	2.0	0.195352	124385	124770	842-842	0.500000	58.4
ndhG-ndhI_p1	ndhG-ndhI	1	TACAAACCAGCGAAGTGACC	AACAAACAGTACGAGCTGCA	20	20	50.0	45.0	58.1	58.1	0.0	1.0	0.178198	125302	126006	1524-1524	0.500000	58.6
ndhG-ndhI_p2	ndhG-ndhI	2	TACAAACCAGCGAAGTGACC	ACAAACAGTACGAGCTGCAA	20	20	50.0	45.0	58.1	58.1	0.0	1.0	0.178198	125302	126006	1523-1523	0.500000	58.6
ndhG-ndhI_p3	ndhG-ndhI	3	TGTGCGGCAGCTACAAAATA	AACAAACAGTACGAGCTGCA	20	20	45.0	45.0	58.2	58.1	10.9	1.0	0.237103	125302	126006	1396-1396	0.500000	58.1
ndhG-ndhI_p4	ndhG-ndhI	4	TGTGCGGCAGCTACAAAATA	ACAAACAGTACGAGCTGCAA	20	20	45.0	45.0	58.2	58.1	10.9	1.0	0.237103	125302	126006	1395-1395	0.500000	58.1
ndhG-ndhI_p5	ndhG-ndhI	5	CACGGTCCAAAGAGGGAAAT	ACAAACAGTACGAGCTGCAA	20	20	50.0	45.0	57.8	58.1	0.0	1.0	0.252268	125302	126006	1495-1495	0.500000	58.0
ndhI_p1	ndhI	1	TCTTGTATATGCTACGTCCCT	ACCAGAAGCAGAGGAGGAAT	21	20	42.9	50.0	55.7	58.0	0.0	0.0	3.350906	126007	126549	1650-1650	0.500000	33.2
ndhI_p2	ndhI	2	TCTTGTATATGCTACGTCCCT	TTGGGGGTGGAATTTATGGC	21	20	42.9	50.0	55.7	58.1	0.0	0.0	3.442118	126007	126549	1740-1740	0.500000	32.5
ndhI_p3	ndhI	3	TCTTGTATATGCTACGTCCCT	TGGTTTTTGGGGGTGGAATT	21	20	42.9	45.0	55.7	57.8	0.0	0.0	3.513225	126007	126549	1746-1746	0.500000	31.9
ndhI_p4	ndhI	4	TCTTGTATATGCTACGTCCCT	GGGTGGAATTTATGGCGTCA	21	20	42.9	50.0	55.7	58.2	0.0	0.0	3.557209	126007	126549	1736-1736	0.500000	31.5
ndhI_p5	ndhI	5	TTCTTGTATATGCTACGTCCCT	ACCAGAAGCAGAGGAGGAAT	22	20	40.9	50.0	56.4	58.0	0.0	0.0	3.592664	126007	126549	1651-1651	0.500000	31.3
ndhI-ndhA_p1	ndhI-ndhA	1	TGCAGCTCGTACTGTTTGTT	ACCAGAAGCAGAGGAGGAAT	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.087911	126550	126642	544-544	0.500000	59.3
ndhI-ndhA_p2	ndhI-ndhA	2	TTGCAGCTCGTACTGTTTGT	ACCAGAAGCAGAGGAGGAAT	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.087911	126550	126642	545-545	0.500000	59.3
ndhI-ndhA_p3	ndhI-ndhA	3	TGCAGCTCGTACTGTTTGTT	TTGGGGGTGGAATTTATGGC	20	20	45.0	50.0	58.1	58.1	8.8	0.0	0.179123	126550	126642	634-634	0.500000	58.6
ndhI-ndhA_p4	ndhI-ndhA	4	TTGCAGCTCGTACTGTTTGT	TTGGGGGTGGAATTTATGGC	20	20	45.0	50.0	58.1	58.1	8.8	0.0	0.179123	126550	126642	635-635	0.500000	58.6
ndhI-ndhA_p5	ndhI-ndhA	5	TGCAGCTCGTACTGTTTGTT	TGGTTTTTGGGGGTGGAATT	20	20	45.0	45.0	58.1	57.8	8.8	0.0	0.250229	126550	126642	640-640	0.500000	58.0
ndhA_p1	ndhA	1	TGCAGCTCGTACTGTTTGTT	CAATGTTACGAGCTTCCGGA	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.056478	126643	128825	2852-2852	0.500000	59.5
ndhA_p2	ndhA	2	TTGCAGCTCGTACTGTTTGT	CAATGTTACGAGCTTCCGGA	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.056478	126643	128825	2853-2853	0.500000	59.5
ndhA_p3	ndhA	3	TGCAGCTCGTACTGTTTGTT	GAGGGAGTGGGCATTATTGG	20	20	45.0	55.0	58.1	58.0	8.8	0.0	0.065013	126643	128825	2907-2907	0.500000	59.5
ndhA_p4	ndhA	4	TGCAGCTCGTACTGTTTGTT	GGGGCTTATCAGGACCAATG	20	20	45.0	55.0	58.1	58.0	8.8	0.0	0.065013	126643	128825	2867-2867	0.500000	59.5
ndhA_p5	ndhA	5	TTGCAGCTCGTACTGTTTGT	GGGGCTTATCAGGACCAATG	20	20	45.0	55.0	58.1	58.0	8.8	0.0	0.065013	126643	128825	2868-2868	0.500000	59.5
ndhH_p1	ndhH	1	TATGACCGCTATTGTTGGCC	TATTCGGGAGCCAAAAACCC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.196155	128827	130008	1633-1633	0.500000	58.4
ndhH_p2	ndhH	2	TCCCTAATGGACCGGCATAT	TATTCGGGAGCCAAAAACCC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.196811	128827	130008	1526-1526	0.500000	58.4
ndhH_p3	ndhH	3	ATTCAGGTCCAATGCGTTGT	TATTCGGGAGCCAAAAACCC	20	20	45.0	50.0	58.1	58.2	17.2	0.0	0.248249	128827	130008	1508-1508	0.500000	58.0
ndhH_p4	ndhH	4	AGGTCCAATGCGTTGTTGTA	TATTCGGGAGCCAAAAACCC	20	20	45.0	50.0	57.7	58.2	17.2	0.0	0.421953	128827	130008	1504-1504	0.500000	56.6
ndhH_p5	ndhH	5	CCGGCATATTCAGGTCCAAT	TATTCGGGAGCCAAAAACCC	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.434680	128827	130008	1515-1515	0.500000	56.5
ndhH-rps15_p1	ndhH-rps15	1	TCCGGAAGCTCGTAACATTG	AGTGCGTGAGTGAGACATTC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.076271	130009	130112	1465-1465	0.500000	59.4
ndhH-rps15_p2	ndhH-rps15	2	CATTGGTCCTGATAAGCCCC	AGTGCGTGAGTGAGACATTC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.084806	130009	130112	1450-1450	0.500000	59.3
ndhH-rps15_p3	ndhH-rps15	3	CCAATAATGCCCACTCCCTC	AGTGCGTGAGTGAGACATTC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.084806	130009	130112	1410-1410	0.500000	59.3
ndhH-rps15_p4	ndhH-rps15	4	TCCGGAAGCTCGTAACATTG	GCGTGAGTGAGACATTCGAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.089949	130009	130112	1462-1462	0.500000	59.3
ndhH-rps15_p5	ndhH-rps15	5	TGCTTCCTCTCCCCCAATAA	AGTGCGTGAGTGAGACATTC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.091879	130009	130112	1423-1423	0.500000	59.3
rps15_p1	rps15	1	CCAATAATGCCCACTCCCTC	AGTGCGTGAGTGAGACATTC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.084806	130113	130391	1410-1410	0.500000	59.3
rps15_p2	rps15	2	TATTGCTTCCTCTCCCCCAA	AGTGCGTGAGTGAGACATTC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.091879	130113	130391	1426-1426	0.500000	59.3
rps15_p3	rps15	3	TGCTTCCTCTCCCCCAATAA	AGTGCGTGAGTGAGACATTC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.091879	130113	130391	1423-1423	0.500000	59.3
rps15_p4	rps15	4	TTATTGCTTCCTCTCCCCCA	AGTGCGTGAGTGAGACATTC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.091879	130113	130391	1427-1427	0.500000	59.3
rps15_p5	rps15	5	TTGCTTCCTCTCCCCCAATA	AGTGCGTGAGTGAGACATTC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.091879	130113	130391	1424-1424	0.500000	59.3
rps15-ycf1_p1	rps15-ycf1	1	ATCAGTCGAAGAACACCGTG	CTAGACGTCGCAGAGAGTTG	20	20	50.0	55.0	57.9	57.8	0.0	10.5	0.232734	130392	131393	1782-1782	0.500000	58.1
rps15-ycf1_p2	rps15-ycf1	2	TTCCCAATTGTTCTGGTGCA	CTAGACGTCGCAGAGAGTTG	20	20	45.0	55.0	57.9	57.8	4.4	10.5	0.245911	130392	131393	1967-1967	0.500000	58.0
rps15-ycf1_p3	rps15-ycf1	3	ATCAGTCGAAGAACACCGTG	ACTAGACGTCGCAGAGAGTT	20	20	50.0	50.0	57.9	58.2	0.0	22.1	0.255034	130392	131393	1783-1783	0.500000	58.0
rps15-ycf1_p4	rps15-ycf1	4	ATCAGTCGAAGAACACCGTG	TGAACCGGTTCGTCTCTCTA	20	20	50.0	50.0	57.9	57.8	0.0	35.4	0.255586	130392	131393	2009-2009	0.500000	58.0
rps15-ycf1_p5	rps15-ycf1	5	ATCAGTCGAAGAACACCGTG	TAGACGTCGCAGAGAGTTGA	20	20	50.0	50.0	57.9	58.2	0.0	10.5	0.261392	130392	131393	1781-1781	0.500000	57.9
ycf1_p1	ycf1	1	TCTTTACCCATCCAACCCCT	ACACGACCGAAAAGCTATCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.074802	135146	135265	1042-1042	0.500000	59.4
ycf1_p2	ycf1	2	AAGTGGCGAATGCACATTTG	ACACGACCGAAAAGCTATCC	20	20	45.0	50.0	57.9	58.0	6.3	0.0	0.074807	135146	135265	953-953	0.500000	59.4
ycf1_p3	ycf1	3	CAAATCCTCGTACCACTCGG	ACACGACCGAAAAGCTATCC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.083400	135146	135265	1301-1301	0.500000	59.3
ycf1_p4	ycf1	4	TCTTTACCCATCCAACCCCT	TGCAGAGGGGTCCATAGAAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.103417	135146	135265	756-756	0.500000	59.2
ycf1_p5	ycf1	5	AAGTGGCGAATGCACATTTG	TGCAGAGGGGTCCATAGAAA	20	20	45.0	50.0	57.9	58.0	6.3	0.0	0.103423	135146	135265	667-667	0.500000	59.2