Hotspot

ndhE-ndhG

SSC · 118976-119205 · 230 bp

Back to Anthriscus

Pi 0.0432

MarkerSeek score 71.20

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 230
variable_sites: 14
pi: 0.0432
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	ACAAGTACATGTTCGAGCATCA	GCGTTAATAGGTGCGATTGC	40.9	50.0	58.1	58.0	273-289 bp	1.0	71.3
ndhE-ndhG_p2	ACAAGTACATGTTCGAGCATCA	ATAGGTGCGATTGCTGTAGC	40.9	50.0	58.1	58.1	267-283 bp	1.0	70.5
ndhE-ndhG_p3	ACAAGTACATGTTCGAGCATCA	CGATTGCTGTAGCTCGTCAA	40.9	50.0	58.1	58.4	260-276 bp	1.0	68.6
ndhE-ndhG_p4	ACAAGTACATGTTCGAGCATCA	AGTTGCGTTAATAGGTGCGA	40.9	45.0	58.1	57.6	277-293 bp	1.0	68.4
ndhE-ndhG_p5	GGCACTCAAAACAAGTACATGT	GCGTTAATAGGTGCGATTGC	40.9	50.0	57.4	58.0	283-299 bp	1.0	67.2