Hotspot

trnL-UAG-ccsA

SSC · 115254-115355 · 102 bp

Back to Anthriscus

Pi 0.0588

MarkerSeek score 74.90

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 102
variable_sites: 9
pi: 0.0588
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	CTTAGGAAGCAGTGCGAGAG	CGATCGAAAAGGAAATATGAGT	55.0	36.4	58.1	54.4	202 bp	1.0	43.6
trnL-UAG-ccsA_p2	CTCTTAGGAAGCAGTGCGAG	CGATCGAAAAGGAAATATGAGT	55.0	36.4	58.1	54.4	204 bp	1.0	43.5
trnL-UAG-ccsA_p3	GTGAAATCGGTAGACACGCT	CGATCGAAAAGGAAATATGAGT	50.0	36.4	58.0	54.4	225 bp	1.0	43.2
trnL-UAG-ccsA_p4	TGAAATCGGTAGACACGCTG	CGATCGAAAAGGAAATATGAGT	50.0	36.4	58.0	54.4	224 bp	1.0	43.2
trnL-UAG-ccsA_p5	AACAAGCCGCTATGGTGAAA	CGATCGAAAAGGAAATATGAGT	45.0	36.4	58.1	54.4	239 bp	1.0	43.0