Hotspot

rps11-rpl36

LSC · 80124-80238 · 115 bp

Back to Anthriscus

Pi 0.0406

MarkerSeek score 74.80

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 115
variable_sites: 7
pi: 0.0406
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CCCCTTTGGTATTCTCCGTG	TTTGTTCCAACCCAAGACAT	55.0	40.0	58.0	55.3	236-237 bp	1.0	61.2
rps11-rpl36_p2	CCCCTTTGGTATTCTCCGTG	ATTTGTTCCAACCCAAGACA	55.0	40.0	58.0	55.3	237-238 bp	1.0	61.1
rps11-rpl36_p3	CCCCTTTGGTATTCTCCGTG	AATTTGTTCCAACCCAAGACA	55.0	38.1	58.0	56.2	238-239 bp	1.0	59.6
rps11-rpl36_p4	CCCCTTTGGTATTCTCCGTG	ATTTGTTCCAACCCAAGACAT	55.0	38.1	58.0	55.9	237-238 bp	1.0	57.8
rps11-rpl36_p5	CCCCTTTGGTATTCTCCGTG	GTTCCAACCCAAGACATAAACA	55.0	40.9	58.0	56.8	233-234 bp	1.0	56.9