Hotspot

psaJ-rpl33

LSC · 67891-68336 · 446 bp

Back to Anthriscus

Pi 0.0297

MarkerSeek score 72.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 19
pi: 0.0297
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	TTTCGAACACAACCGGTACA	45.0	45.0	58.0	57.7	541-543 bp	1.0	83.1
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	TTTCGAACACAACCGGTACA	45.0	45.0	58.1	57.7	532-534 bp	1.0	82.8
psaJ-rpl33_p3	ACATATCTTTCCGTGGCACC	TTTCGAACACAACCGGTACA	50.0	45.0	58.0	57.7	620-622 bp	1.0	82.3
psaJ-rpl33_p4	TTCCCAGATGCGTTGACATT	TTTCGAACACAACCGGTACA	45.0	45.0	57.8	57.7	539-541 bp	1.0	81.7
psaJ-rpl33_p5	TTTCCCAGATGCGTTGACAT	TTTCGAACACAACCGGTACA	45.0	45.0	57.8	57.7	540-542 bp	1.0	81.7