Hotspot

psbE-petL

LSC · 65461-66592 · 1132 bp

Back to Anthriscus

Pi 0.0308

MarkerSeek score 66.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1132
variable_sites: 51
pi: 0.0308
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCAGTGCTTC	ACCAAAATAACTAGTTATAGTAGGCA	50.0	30.8	57.8	56.0	1183-1186 bp	1.0	43.9
psbE-petL_p2	CAACCCGCAATGAATAGGGA	ACCAAAATAACTAGTTATAGTAGGCA	50.0	30.8	57.9	56.0	1260-1263 bp	1.0	43.6
psbE-petL_p3	ACTGACGAATAACCAACCCG	ACCAAAATAACTAGTTATAGTAGGCA	50.0	30.8	57.9	56.0	1273-1276 bp	1.0	43.6
psbE-petL_p4	ACGAATAACCAACCCGCAAT	ACCAAAATAACTAGTTATAGTAGGCA	45.0	30.8	58.2	56.0	1269-1272 bp	1.0	43.6
psbE-petL_p5	CGAATAACCAACCCGCAATG	ACCAAAATAACTAGTTATAGTAGGCA	50.0	30.8	57.8	56.0	1268-1271 bp	1.0	43.6