Hotspot

ycf4-cemA

LSC · 61153-61835 · 683 bp

Back to Anthriscus

Pi 0.0381

MarkerSeek score 69.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 38
pi: 0.0381
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AATCGAACAAAAAGCTGCCG	TTGGAGCGTTTCTTTGTGGA	45.0	45.0	57.9	58.0	1017-1026 bp	1.0	83.9
ycf4-cemA_p2	CGAACAAAAAGCTGCCGAAT	TTGGAGCGTTTCTTTGTGGA	45.0	45.0	57.9	58.0	1014-1023 bp	1.0	83.9
ycf4-cemA_p3	CGAACAAAAAGCTGCCGAAT	GGAATTCCTGAGCCCAAGAG	45.0	55.0	57.9	58.0	1156-1165 bp	1.0	83.8
ycf4-cemA_p4	AATCGAACAAAAAGCTGCCG	GGAATTCCTGAGCCCAAGAG	45.0	55.0	57.9	58.0	1159-1168 bp	1.0	83.7
ycf4-cemA_p5	AAATCGTCGCATCTTCCTCC	TTGGAGCGTTTCTTTGTGGA	50.0	45.0	58.1	58.0	1176-1185 bp	1.0	83.2