Hotspot

trnE-UUC-trnT-GGU

LSC · 31537-32249 · 713 bp

Back to Anthriscus

Pi 0.0361

MarkerSeek score 69.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 713
variable_sites: 35
pi: 0.0361
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CTGACTATCGTGCTCCCAAC	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	1313-1384 bp	1.0	86.7
trnE-UUC-trnT-GGU_p2	CCCAGGGAAATAGGAAGGGA	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	1028-1096 bp	1.0	86.5
trnE-UUC-trnT-GGU_p3	TCCCAGGGAAATAGGAAGGG	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	1029-1097 bp	1.0	86.5
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	756-817 bp	1.0	86.4
trnE-UUC-trnT-GGU_p5	CTGACTATCGTGCTCCCAAC	CCGATGACTTACGCCTTACC	55.0	55.0	58.1	58.2	1310-1381 bp	1.0	85.6