Hotspot

psbM-trnD-GUC

LSC · 30451-31137 · 687 bp

Back to Anthriscus

Pi 0.0361

MarkerSeek score 74.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 37
pi: 0.0361
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGCAGAAAAGCAGTAGGAACT	CCCTTCCTATTTCCCTGGGA	42.9	55.0	57.2	58.1	842-845 bp	1.0	68.9
psbM-trnD-GUC_p2	AGCAGAAAAGCAGTAGGAACT	TCCCTTCCTATTTCCCTGGG	42.9	55.0	57.2	58.1	843-846 bp	1.0	68.9
psbM-trnD-GUC_p3	AAGCAGAAAAGCAGTAGGAACT	CCCTTCCTATTTCCCTGGGA	40.9	55.0	57.9	58.1	843-846 bp	1.0	66.4
psbM-trnD-GUC_p4	AAGCAGAAAAGCAGTAGGAACT	TCCCTTCCTATTTCCCTGGG	40.9	55.0	57.9	58.1	844-847 bp	1.0	66.4
psbM-trnD-GUC_p5	AGCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.2	58.5	816-819 bp	1.0	66.0