Hotspot

rpoB-trnC-GCA

LSC · 27106-28343 · 1238 bp

Back to Anthriscus

Pi 0.0297

MarkerSeek score 65.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1238
variable_sites: 54
pi: 0.0297
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCGCCTACACAAGCAAATTG	CTTACCACTCGGCCATATCG	50.0	55.0	58.0	57.9	1941-1964 bp	1.0	83.0
rpoB-trnC-GCA_p2	CCGCCTACACAAGCAAATTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1981-2004 bp	1.0	82.8
rpoB-trnC-GCA_p3	CCGCCTACACAAGCAAATTG	ACCTAATGCACAACCCGTTT	50.0	45.0	58.0	58.0	2107 bp	0.333333	53.1
rpoB-trnC-GCA_p4	AATCTTCCTCCCCAATCCGA	ACCTAATGCACAACCCGTTT	50.0	45.0	58.1	58.0	1876 bp	0.333333	52.4
rpoB-trnC-GCA_p5	ATCTTCCTCCCCAATCCGAT	ACCTAATGCACAACCCGTTT	50.0	45.0	57.9	58.0	1875 bp	0.333333	52.3