Hotspot

atpH-atpI

LSC · 13590-14730 · 1141 bp

Back to Anthriscus

Pi 0.0257

MarkerSeek score 75.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1141
variable_sites: 44
pi: 0.0257
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	1203-1213 bp	1.0	82.1
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.9	57.9	1259-1269 bp	1.0	81.4
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	57.9	57.9	1219-1229 bp	1.0	81.2
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.2	57.9	1204-1214 bp	1.0	80.9
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	ATAGGGGAATCCATGGAGGG	55.0	55.0	58.2	57.9	1263-1273 bp	1.0	80.7