Hotspot

rps16-trnQ-UUG

LSC · 5668-7099 · 1432 bp

Back to Anthriscus

Pi 0.0337
MarkerSeek score 73.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1432
variable_sites
70
pi
0.0337
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1551-1572 bp 1.0 83.8
rps16-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1552-1573 bp 1.0 83.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA AAATATAGATGGGGCGTGGC 45.0 50.0 58.1 57.8 1562-1583 bp 1.0 83.0
rps16-trnQ-UUG_p4 CGGATCGTGTCCTTCAAGTC AAATATAGATGGGGCGTGGC 55.0 50.0 58.4 57.8 1577-1598 bp 1.0 81.1
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG AAATATAGATGGGGCGTGGC 55.0 50.0 58.4 57.8 1576-1597 bp 1.0 81.1