Hotspot

rrn16

IRb · 91347-92839 · 1493 bp

Back to Anthoxanthum

Pi 0.0208

MarkerSeek score 60.60

Primer available yes

Label rrn16

Feature Metadata

label_name: rrn16
feature_type: rRNA
region: IRb
length_bp: 1493
variable_sites: 31
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16_p1	CCGAATCCGCTTTGTCTACA	TTTTGGGCGTGAAGAAGTGT	50.0	45.0	58.0	58.2	1606-1608 bp	1.0	79.7
rrn16_p2	CCGAATCCGCTTTGTCTACA	GCGTGAAGAAGTGTCAAACC	50.0	50.0	58.0	57.6	1600-1602 bp	1.0	78.5
rrn16_p3	CCGAATCCGCTTTGTCTACA	GGCGTGAAGAAGTGTCAAAC	50.0	50.0	58.0	57.6	1601-1603 bp	1.0	78.5
rrn16_p4	TCCGAATCCGCTTTGTCTAC	TTTTGGGCGTGAAGAAGTGT	50.0	45.0	57.7	58.2	1607-1609 bp	1.0	77.4
rrn16_p5	ATTCCGAATCCGCTTTGTCT	TTTTGGGCGTGAAGAAGTGT	45.0	45.0	57.6	58.2	1609-1611 bp	1.0	76.5