Hotspot

rpl32-trnL-UAG

SSC · 104701-105331 · 631 bp

Back to Anthoxanthum

Pi 0.016
MarkerSeek score 54.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
631
variable_sites
10
pi
0.016
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGGCAACAACAACAAACAA TTCGAACCGAGATGCTTGAG 45.0 50.0 57.9 58.0 719-725 bp 1.0 81.2
rpl32-trnL-UAG_p2 ACATCCAAAACCAAAGGGCT TTCGAACCGAGATGCTTGAG 45.0 50.0 57.9 58.0 743-749 bp 1.0 81.1
rpl32-trnL-UAG_p3 GGGGCAACAACAACAAACAA GGATTCGAACCGAGATGCTT 45.0 50.0 57.9 58.1 722-728 bp 1.0 80.7
rpl32-trnL-UAG_p4 GGGGCAACAACAACAAACAA GATCCGCACAAAAGAGTTGC 45.0 50.0 57.9 58.0 1271-1277 bp 1.0 80.1
rpl32-trnL-UAG_p5 ACATCCAAAACCAAAGGGCT GATCCGCACAAAAGAGTTGC 45.0 50.0 57.9 58.0 1295-1301 bp 1.0 80.1