Hotspot

rps8-rpl14

LSC · 76206-76348 · 143 bp

Back to Anthoxanthum

Pi 0.028

MarkerSeek score 68.60

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 143
variable_sites: 4
pi: 0.028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TCAGCAATAGTGTCCTTGCC	CGATTGCCGAGGAATTGAGA	50.0	50.0	57.9	58.1	234 bp	1.0	80.0
rps8-rpl14_p2	TCAGCAATAGTGTCCTTGCC	ATCCAAAAGGAACTCGCGTT	50.0	45.0	57.9	58.1	261 bp	1.0	79.5
rps8-rpl14_p3	TCAGCAATAGTGTCCTTGCC	AATCCAAAAGGAACTCGCGT	50.0	45.0	57.9	58.1	262 bp	1.0	79.4
rps8-rpl14_p4	GCAATAGTGTCCTTGCCCAT	CGATTGCCGAGGAATTGAGA	50.0	50.0	58.2	58.1	231 bp	1.0	79.0
rps8-rpl14_p5	GCAATAGTGTCCTTGCCCAT	ATCCAAAAGGAACTCGCGTT	50.0	45.0	58.2	58.1	258 bp	1.0	78.5