Hotspot

rpl33-rps18

LSC · 64528-64808 · 281 bp

Back to Anthoxanthum

Pi 0.00719

MarkerSeek score 44.50

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 281
variable_sites: 2
pi: 0.00719
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TCGCAAGCATACGACTCATC	GAAAAGGTTGTTTAGATGTATACA	50.0	29.2	58.2	53.1	340-343 bp	1.0	41.2
rpl33-rps18_p2	CGCAAGCATACGACTCATCA	GAAAAGGTTGTTTAGATGTATACA	50.0	29.2	58.4	53.1	339-342 bp	1.0	41.2
rpl33-rps18_p3	AGAATCGCCACAATACACCC	GAAAAGGTTGTTTAGATGTATACA	50.0	29.2	58.0	53.1	395-398 bp	1.0	41.0
rpl33-rps18_p4	CCACAATACACCCGGACAAT	GAAAAGGTTGTTTAGATGTATACA	50.0	29.2	57.9	53.1	388-391 bp	1.0	41.0
rpl33-rps18_p5	AGAATCGCCACAATACACCC	GAAAAGGTTGTTTAGATGTATACAT	50.0	28.0	58.0	53.6	395-398 bp	1.0	41.0