Hotspot

psaJ-rpl33

LSC · 63895-64326 · 432 bp

Back to Anthoxanthum

Pi 0.00248

MarkerSeek score 61.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 432
variable_sites: 1
pi: 0.00248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TATCTCTCTGTAGCACCCGT	GAAATCCCCGTCGATTCCTC	50.0	55.0	57.3	58.1	608-635 bp	1.0	76.8
psaJ-rpl33_p2	TATCTCTCTGTAGCACCCGT	GGCACCTTTTCGAACACAAC	50.0	50.0	57.3	58.2	585-612 bp	1.0	76.7
psaJ-rpl33_p3	TATCTCTCTGTAGCACCCGT	CGATTCCTCATTGGCACCTT	50.0	50.0	57.3	58.2	597-624 bp	1.0	76.0
psaJ-rpl33_p4	ATCTCTCTGTAGCACCCGTG	GAAATCCCCGTCGATTCCTC	55.0	55.0	58.9	58.1	607-634 bp	1.0	75.5
psaJ-rpl33_p5	ATCTCTCTGTAGCACCCGTG	GGCACCTTTTCGAACACAAC	55.0	50.0	58.9	58.2	584-611 bp	1.0	75.4