Hotspot

rbcL-psaI

LSC · 55848-56797 · 950 bp

Back to Anthoxanthum

Pi 0.017

MarkerSeek score 69.30

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 950
variable_sites: 16
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TTCGAGCCGGTAGATACCAT	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.0	57.9	1043-1051 bp	1.0	81.1
rbcL-psaI_p2	AGCCGGTAGATACCATCGAT	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.8	57.9	1039-1047 bp	1.0	79.5
rbcL-psaI_p3	TTCGAGCCGGTAGATACCAT	GAAGTCATTGCAATTGCCGG	50.0	50.0	58.0	58.4	1046-1054 bp	1.0	78.8
rbcL-psaI_p4	AGCTTGCAAATGGAGTCCTG	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.5	57.9	1107-1115 bp	1.0	77.4
rbcL-psaI_p5	CAGCTTGCAAATGGAGTCCT	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.5	57.9	1108-1116 bp	1.0	77.4