Hotspot

ycf3-trnS-GGA

LSC · 43689-44251 · 563 bp

Back to Anthoxanthum

Pi 0.0124

MarkerSeek score 62.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 563
variable_sites: 7
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	746 bp	1.0	79.0
ycf3-trnS-GGA_p2	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTCAA	50.0	45.0	58.1	57.8	696 bp	1.0	78.8
ycf3-trnS-GGA_p3	TCTCCTGAGGTTGTCGGAAT	AGTTCCAATGCTACGCCTTC	50.0	50.0	58.1	58.3	698 bp	1.0	78.2
ycf3-trnS-GGA_p4	TTCTCCTGAGGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	747 bp	1.0	77.2
ycf3-trnS-GGA_p5	TTTCTCCTGAGGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	748 bp	1.0	77.2