Hotspot

trnG-GCC-trnM-CAU

LSC · 12140-12609 · 470 bp

Back to Anthoxanthum

Pi 0.0181

MarkerSeek score 65.90

Primer available yes

Label trnG-GCC-trnM-CAU

Feature Metadata

label_name: trnG-GCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 8
pi: 0.0181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	GCGAGCGTAGTTCAATGGTA	CTAGAGCGGAGTAGAGCAGT	50.0	55.0	58.1	58.0	595-622 bp	1.0	83.8
trnG-GCC-trnM-CAU_p2	CGTAGCGAGCGTAGTTCAAT	CTAGAGCGGAGTAGAGCAGT	50.0	55.0	58.2	58.0	599-626 bp	1.0	83.0
trnG-GCC-trnM-CAU_p3	TAGCGAGCGTAGTTCAATGG	CTAGAGCGGAGTAGAGCAGT	50.0	55.0	57.8	58.0	597-624 bp	1.0	82.8
trnG-GCC-trnM-CAU_p4	GCGAGCGTAGTTCAATGGTA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.1	57.8	564-591 bp	1.0	82.5
trnG-GCC-trnM-CAU_p5	CGACCCAAGTGGATATACGC	CTAGAGCGGAGTAGAGCAGT	55.0	55.0	58.2	58.0	633-660 bp	1.0	82.4