Hotspot

clpP

LSC · 71551-72972 · 1422 bp

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Pi 0.0255

MarkerSeek score 59.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 36
pi: 0.0255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGCACCAACGGAAACCATAA	TCCAAAAACCCGGATTCGTT	45.0	45.0	58.0	58.0	2561 bp	1.0	81.5
clpP_p2	TTGCACCAACGGAAACCATA	TCCAAAAACCCGGATTCGTT	45.0	45.0	58.0	58.0	2562 bp	1.0	81.5
clpP_p3	GATTGGCTGTCTTGCGTTTC	TCCAAAAACCCGGATTCGTT	50.0	45.0	58.0	58.0	2331 bp	1.0	81.5
clpP_p4	TGCACCAACGGAAACCATAA	AGCAAAGGCCAGAAAAGACA	45.0	45.0	58.0	57.9	2609 bp	1.0	81.0
clpP_p5	TTGCACCAACGGAAACCATA	AGCAAAGGCCAGAAAAGACA	45.0	45.0	58.0	57.9	2610 bp	1.0	81.0